Cov txheej txheem:
Video: Tus Nqi Qis Tsawg Tus Nqi thiab Brightfield Microscopes: 9 Kauj Ruam (nrog Duab)
2024 Tus sau: John Day | [email protected]. Kawg hloov kho: 2024-01-30 09:26
Fusion 360 Cov Haujlwm »
Fluorescence microscopy yog ib qho qauv siv los ua kom pom cov qauv tshwj xeeb hauv kev lom neeg thiab lwm yam kev kuaj lub cev. Cov khoom txaus siab hauv cov qauv (piv txwv li cov paj hlwb, cov hlab ntshav, mitochondria, thiab lwm yam) yog pom tau vim tias cov tshuaj fluorescent sib txuas tsuas yog cov qauv tshwj xeeb. Qee cov duab me me zoo nkauj tshaj plaws tau sau nrog lub tsom iav fluorescence; txheeb xyuas cov duab no nthuav tawm ntawm Nikon MicroscopyU lub vev xaib kom pom qee qhov piv txwv. Fluorescence microscopy muaj txiaj ntsig zoo rau ntau yam kev tshawb fawb txog biology uas tsom mus rau cov qauv tshwj xeeb lossis hom cell. Piv txwv li, ntau qhov kev tshawb fawb tshawb fawb txog neurons hauv lub paj hlwb vam khom siv cov txheej txheem fluorescence microscopy uas tshwj xeeb cov duab neurons.
Hauv cov lus qhia no, kuv yuav mus hla cov hauv paus ntsiab lus ntawm tsom iav tsom iav thiab yuav ua li cas tsim peb qhov sib txawv tus nqi qis fluorescence tsom iav. Cov kab ke no feem ntau raug nqi ntau txhiab daus las, tab sis tsis ntev los no tau siv zog ua kom lawv nkag tau yooj yim dua. Cov qauv tsim uas kuv nthuav qhia ntawm no siv lub xov tooj ntse, dSLR, thiab USB tsom iav. Txhua qhov kev tsim qauv no tseem ua haujlwm zoo li lub tshuab tsom iav zoo nkauj. Cia peb pib!
Kauj Ruam 1: Txheej Txheem Txheej Txheem Me Me
Txhawm rau nkag siab lub tswv yim yooj yim ntawm tsom iav tsom iav, xav txog lub hav zoov tuab thaum hmo ntuj uas muaj cov ntoo, tsiaj, hav txwv yeem, thiab txhua yam ntxiv nyob hauv hav zoov. Yog tias koj teeb lub teeb nyem rau hauv hav zoov koj pom txhua yam ntawm cov qauv no thiab nws tuaj yeem nyuaj rau pom kev tshwj xeeb rau tsiaj lossis nroj tsuag. Cia peb hais tias koj tsuas yog xav pom blueberry hav txwv yeem hauv hav zoov. Txhawm rau ua kom tiav qhov no, koj qhia cov foob pob hluav taws kom nyiam rau tsuas yog tsob ntoo blueberry, yog li ntawd tsuas yog tsob ntoo blueberry pom thaum koj saib mus rau tom hav zoov. Koj tuaj yeem hais tias koj tau sau cov ntoo blueberry nrog cov paj tawg kom koj tuaj yeem pom tsuas yog cov ntoo blueberry hauv hav zoov.
Hauv qhov kev sib piv no, hav zoov sawv cev rau tag nrho cov qauv, blueberry hav txwv yeem sawv cev rau cov qauv koj xav pom (piv txwv li cov xov tooj tshwj xeeb lossis cov kab mob subcellular organelle), thiab cov nplaim taws yog cov tshuaj fluorescent. Cov ntaub ntawv uas koj ci lub teeb nyem ib leeg yam tsis muaj lub foob pob hluav taws yog qhov zoo ib yam rau lub tshuab tsom ci ci.
Cov kauj ruam tom ntej yog nkag siab txog kev ua haujlwm yooj yim ntawm cov tshuaj fluorescent (tseem hu ua fluorophores). Fluorophores yog cov khoom me me tiag tiag (ntawm qhov ntsuas ntawm nanometers) tsim los txuas rau cov qauv tshwj xeeb hauv cov qauv. Lawv nqus lub teeb hla qhov nqaim ntawm cov nthwv dej thiab rov tso tawm lwm lub nthwv dej ntawm lub teeb. Piv txwv li, ib tus fluorophore tuaj yeem nqus tau lub teeb xiav (piv txwv li fluorophore zoo siab los ntawm lub teeb xiav) thiab tom qab ntawd rov tso lub teeb ntsuab. Feem ntau qhov no suav nrog qhov ua rau muaj kev txaus siab thiab tso tawm ntau ntau (daim duab saum toj no). Cov kab ntawv no qhia qhov nthwv dej ntawm lub teeb uas cov tshuaj fluorophore nqus tau thiab lub nthwv dej ntawm lub teeb uas lub fluorophore tso tawm.
Kev tsim lub tsom iav zoo ib yam zoo ib yam li lub tshuab tsom iav zoo ib yam nrog ob qhov sib txawv loj. Ua ntej, lub teeb kom pom qhov piv txwv yuav tsum yog lub nthwv dej uas ua rau muaj kev zoo siab fluorophore (piv txwv li saum toj no, lub teeb yog xiav). Qhov thib ob, lub tshuab tsom iav xav tau sau tsuas yog lub teeb tso tawm (lub teeb ntsuab), thaum thaiv qhov xiav. Qhov no yog vim tias lub teeb xiav mus rau txhua qhov tab sis lub teeb ntsuab tuaj tsuas yog los ntawm cov qauv tshwj xeeb hauv cov qauv. Txhawm rau thaiv lub teeb xiav, lub tsom iav feem ntau muaj qee yam hu ua lub tshuab lim ntev uas cia lub teeb ntsuab hla mus yam tsis muaj lub teeb xiav. Txhua lub lim dej hla dhau muaj qhov txiav tawm nthwv dej. Yog tias lub teeb muaj lub nthwv dej ntev dua li kev txiav tawm, tom qab ntawd nws tuaj yeem hla dhau lub lim. Yog li lub npe, "hla dhau." Ntev wavelengths raug thaiv.
Nov yog ntau qhov kev tshuaj xyuas ntawm lub tshuab tsom iav fluorescence:
bitesizebio.com/33529/fluorescence-microsc… nyeem ntxiv
www.microscopyu.com/techniques/fluorescenc…
www.youtube.com/watch?v=PCJ13LjncMc
Kauj Ruam 2: Ua Qauv Microscopes Nrog Ray Optics
Khiav Tawm Hauv Kev Sib Tw Optics
Pom zoo:
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