Ua Nrog Zej Zos Schizophyllum: Tsim Kom Muaj Kab Mob Zoo Los Ntawm Pom Mushroom: 3 Kauj Ruam (nrog Duab)

2024 Tus sau: John Day | [email protected]. Kawg hloov kho: 2024-01-30 09:29

Cov lus qhia no yog tsom mus rau kev piav qhia yuav ua li cas thiaj tsim tau kab lis kev cai tsis huv ntawm cov nceb Mushroom Schizophyllum (npe hu ua Split Gill mushroom) ntawm cov tais zaub uas siv pom cov nceb. Cov zej zog Schizophyllum tau pom muaj ntau dua 28, 000 tus poj niam txiv neej, tuaj yeem pom nyob hauv txhua lub teb chaws tshwj tsis yog Antarctica, thiab muaj peev xwm (zoo li lwm yam "dawb thiab xim av rot fungi") txhawm rau ua rau cov qog endocrine cuam tshuam kev ua qias tuaj (plastics, hormone imimicking industrial petrochemicals zoo li cov chiv Atrazine, cov yas yas Bisphenol-A, lossis cov tshuaj hluavtaws hormone Diethylstilbestrol).

Kuv yuav mus hla cov txheej txheem qhia txog cov nceb qus, yuav ua li cas ua kom cov zaub mov muaj txiaj ntsig zoo agar petri, thiab yuav siv koj li cas pom cov nceb kom loj hlob tsis huv nyob hauv koj lub tais zaub mov. Lwm cov lus qhia hauv kab ntawv no hla cov txheej txheem xws li kev txheeb xyuas caj ces ntawm cov nceb, kev tshuaj ntsuam yooj yim enzymatic los ntsuas cov nceb muaj peev xwm zom cov yas thiab cov tshuaj hormones ua raws cov pa phem, thiab yuav ua li cas ua cheese nrog S. Commune.

Txoj haujlwm no tau tawm los ntawm kev sib koom tes ua haujlwm ntawm Mary Maggic, Paula Pin, thiab kuv tus kheej thaum nyob ntawm HANGAR hauv Barcelona Spain thaum lub Cuaj Hlis 2018. Xav paub ntau ntxiv txog qhov project nyeem ntawm ~~ lossis hla tag nrho Bla Bla thiab mus ncaj nraim rau Kauj Ruam 1 rau kev qhia:)

Cov duab los ntawm Schizophyllum Township Fanzine ~ cov ntawv tuaj yeem rub tawm dawb ntawm no

Thaum nyob hauv peb peb lub hom phiaj tseem ceeb yog:

1 ~ Tshawb nrhiav thiab zom cov ntaub ntawv tshawb fawb ntawm Schizophyllum Kev Sib Tham thiab nws muaj ntau yam kev cuam tshuam nrog cov tshuaj hormones thiab endocrine cuam tshuam kev ua qias neeg petrochemical. S. Commune yog ib qho ntawm ntau yam "dawb thiab xim av rot" nrog lub peev xwm los zom cov tshuaj hormones thiab endocrine cuam tshuam kev ua qias tuaj. Cov fungi no tawm ntawm cov ntoo los ntawm kev tso tawm "lignocellulosic" enzymes los ntawm lawv lub cev sib txuas lossis "mycelia" thiab cov enzymes muaj peev xwm zom cov cellulose thiab lignin uas cov ntoo ua los ntawm. Vim tias cov qauv zoo sib xws ntawm cellulose, lignin, thiab EDS ' / Hormones zoo li Atrazine, BPA, Estradiol, Diethylstilbestrol, thiab Nonylphenols, "lignocellulosic" enzymes zais los ntawm cov kab mob tuaj yeem ua rau ntau yam tshuaj lom ntau ntxiv. Ntxiv rau qhov no, S. Commune tsim cov tshuaj tiv thaiv kab mob polysaccharides uas muaj txiaj ntsig zoo li kev tiv thaiv kab mob qog noj ntshav thiab kev kho mob (Pom zoo hauv Nyij Pooj rau kev kho mob qog noj ntshav ntawm ncauj tsev menyuam), tuaj yeem txhim kho phytoestrogenic cov yam ntxwv ntawm qee yam nroj tsuag los ntawm kev fermentation, tuaj yeem siv qhov chaw rennet thiab lactobacilius hauv cov txheej txheem cheesemaking, thiab tsim cov thrombin clot dissolving enzymes (muaj txiaj ntsig zoo rau kev kho mob thrombosis). Coj txawv txawv, tib yam "beta-glucans" uas tuaj yeem muab rho tawm nrog dej kub ua kev kho mob qog noj ntshav thiab tshuaj tiv thaiv kab mob, kuj tseem siv los ntawm kev lag luam roj av txhawm rau txhim kho cov txiaj ntsig roj, khi S. Commune hauv cov lus tsis txaus ntseeg lub voj rau kev tsim khoom ntawm endocrine cuam tshuam petrochemicals nws muaj peev xwm degrade thiab kho tau.

2 ~ Tsim DIY thiab DIWO (ua nrog lwm tus) cov txheej txheem uas ua kom yooj yim cov kev txais no rau kev ua haujlwm nrog EDC's thiab cov kab mob los txhais cov kev paub paub txog kev tshawb fawb codified rau hauv hom lus uas nkag tau yooj yim dua. Siv cov ntawv txais no rau kev kawm, rau kev nug, rau kev tawm tsam rau kev tsim cov txheej txheem molecular, thiab tsim cov lus piav qhia tshiab txog ntau yam sib txawv los ua thiab peb cov kev sib koom ua ke.

3 ~ Sib tw qhov tseem ceeb cisheteronormative, queerphobic hais lus ib puag ncig endocrine cuam tshuam rau tshuaj (EDC's). Txhawm rau hais Malin Ah-King thiab Eva Hayward cov nyiaj kawm ntawv ntawm cov teeb meem, Ntau qhov xov xwm tshaj tawm tau tshaj tawm cov lus dab neeg txaus ntshai no los ntawm peb lub tiaj nraum qaum tsev. Tsis yog hais txog ntau yam lwm yam kev pheej hmoo noj qab haus huv cuam tshuam nrog kev raug lom, qhov teeb meem zoo tshaj plaws thiab kev hais lus sawv hauv kev sib cav thiab cov lus teb tseem ceeb. thiab txawm tuag los? Cov kab lis kev cai dab tsi (ntau yam uas tau hloov pauv thoob ntiaj teb), tau tshwm sim? (Tshuaj lom poj niam).

Kev sib tham hauv Schizophyllum yog qhov tshwj xeeb "dawb rot fungi" hauv qhov nws tau pom tias muaj ntau dua 28, 000 tus poj niam txiv neej. Qhov kev hloov pauv no, ntau qhov theorize, tau ua rau muaj kev sib txawv ntau yam tsis txaus ntseeg ntawm hom tsiaj no uas muaj nyob hauv txhua lub teb chaws thoob ntiaj teb tshwj tsis yog Antarctica. Peb xav ua kev zoo siab kev sib daj sib deev zoo nkauj ntawm cov kab mob no raws li ib qho piv txwv ntawm cov hav zoov ntawm biology. Kev hais lus tseem ceeb nyob ib puag ncig EDC hauv kev tshaj xov xwm, thiab txawm tias los ntawm ib puag ncig cov neeg ua haujlwm tsom mus rau qhov kev piav qhia tsis tseem ceeb, tias cov qauv no ua rau qee yam kev sib deev lossis poj niam txiv neej uas peb lub zej zog muaj kab mob, sau tsis yog tus qauv, thiab feem ntau nrhiav kev tshem tawm lossis zam. Tsis yog tsuas yog kev sib deev-chim siab, queer phobic tsom mus rau pej xeem kev yws txog EDC qhov kev tshawb fawb tsis muaj tseeb (cov tshuaj hormones thiab endocrine cuam tshuam tsis ua rau tib neeg queer lossis trans) tab sis nws tsis quav ntsej tag nrho cov kev puas tsuaj tiag tiag uas peb tuaj yeem txuas rau EDC's: rog, mob qog noj ntshav, qee yam mob qog noj ntshav, nrog rau lwm yam kev pheej hmoo noj qab haus huv.

Kauj Ruam 1: Nrhiav Kev Sib Tham Schizophyllum Nyob Hauv Koj Lub Zej Zog

Cov qib qib tsis huv ntawm haiv neeg ntawm Schizophyllum Kev Sib Tham tuaj yeem yuav tau ib puag ncig $ 350 los ntawm lub txhab nyiaj kab lis kev cai microbiological xws li ATCC …

Kuv tau yooj yim vim tias kuv tau nyeem txog Schizophyllum Kev Sib Tham thiab nyuam qhuav tshwm sim pom tias nws loj hlob ntawm tsob ntoo nyob rau pem hauv ntej ntawm kuv chav tsev nyob ib hnub. Yog tias koj tsis muaj hmoo kom yuam kev li S. Commune hauv koj lub zej zog, koj yuav xav mus rau thaj chaw ntoo kom tshawb nrhiav "bracket" nceb ntawm cov ntoo tawv ntoo (tsob ntoo zoo li lawv tsis ua zoo heev) lossis ntoo uas poob. S. Commune yog ib qho ntawm cov kab mob ntau tshaj plaws nyob ib puag ncig lub ntiaj teb thiab tuaj yeem pom pom nyob txhua qhov chaw uas muaj cov ntoo tawv kom loj tuaj.

Mushroomobserver.org muab cov ncauj lus ntxaws ntxaws txog qhov twg thiab thaum twg koj yuav pom S. Commune, ntxiv rau yuav txheeb xyuas nws li cas thiab yuav ua li cas zam kev "zoo ib yam" uas yuav ua rau pom qhov tsis raug:

"QHOV TSEEB TSHAJ PLAWS: Schizophyllum zej zog tau txheeb xyuas yooj yim los ntawm nws cov plaub hau sib cais, cov plaub hau tuab, thiab lub ntsej muag zoo li lub kaus mom. Ib qho zoo sib xws hu ua "Crimped Gill" Plicaturopsis crispa tsis nthuav dav li.

HABITAT: Schizophyllum zej zog loj hlob ntawm cov ntoo tawv siv cov enzymes los txo qis lignin thiab ua rau "dawb rot." Yog li nws tau pom nyob hauv txhua qhov chaw ntoo. Nws tuaj yeem pom nyob rau xyoo puag ncig vim nws muaj peev xwm ua kom qhuav, ua kom cov dej nyob hauv cov xwm txheej zoo dua, thiab tom qab ntawd txuas ntxiv mus.

Cov kab mob no tuaj yeem ua kab mob rau ntawm cov ntoo me me uas muaj kev nyuaj siab los ntawm lwm yam, xws li kev qhuav.

LOOK ALIKES: Plicaturopsis crispa lossis "Crimped Gill" zoo ib yam li Split Gill. Nws kuj muaj lub plhaub/kiv cua zoo li lub cev txiv hmab txiv ntoo. Txawm li cas los xij lub hau yog xim av xim av lossis xim av daj, thiab sab hauv qab yog qhov txawv heev. Nws tau ua rau cov hlab ntshav zoo li cov kab noj hniav uas muaj ntau yam sib txawv dua li cov hniav sib cais ntawm Schizophyllum lub zej zog. Crimped Gill tsis tshua muaj tshwm sim tab sis txawv thaum pom."

Kauj Ruam 2: Ua Cov Khoom Noj Cov Khoom Noj Agar rau S. Commune kom Loj Hlob Rau

Txhawm rau cais cov kab lis kev cai ntawm S. Commune koj tau pom koj yuav xav tau qee cov tais diav uas muaj cov zaub mov rau nceb. Malt extract Agar yog ib qho yooj yim los ua zaub mov rau S. Commune, lossis "as -ham" uas tuaj yeem ua nrog cov khoom xyaw yooj yim. Nws tseem yog cov zaub mov zoo siv thaum ua qhov kev sim tshuaj enzymatic nrog S. Commune thiab lwm yam kab mob dawb los yog xim av rot ~~ cov txheej txheem no yuav raug them rau hauv lwm cov lus qhia.

NCO TSEG CEEB TOOM: Yog tias koj muaj teeb meem nrhiav S. Commune, muaj ntau ntau cov kab mob hu ua fungi hauv tib tsev neeg uas tuaj yeem ua kab lis kev cai siv tib cov txheej txheem thiab zoo ib yam yog tias tsis zoo dua ntawm kev ua rau cov kab mob hauv lub cev puas tsuaj. Qaib Cov Txwv Tail (Trametes Versicolor) tau pom nyob hauv ntau qhov kev tshawb fawb uas saib rau ntau pua ntawm cov kab dawb thiab xim av rot los ua cov khoom lag luam siab tshaj plaws ntawm "lignocellulosic" enzymes uas ua rau cov pa phem phem phem. Oyster nceb kuj zoo heev ntawm qhov no.

Kuv ua kuv txoj haujlwm microbiological hauv zej zog ib puag ncig kev sim tshuaj hu ua BUGSS (Baltimore Underground Science Space) uas kuv tau nkag mus rau iav iav, nplai digital, kawm tiav lub tog raj kheej, autoclave (rau cua sov thiab siab kom tsis muaj menyuam ntawm kev tshaj xov xwm), thiab lwm yam Labs zoo li no muaj tau tshwm sim thoob plaws ntiaj teb hauv kaum xyoo dhau los, yog li koj yuav xav tshawb xyuas cov npe no seb puas muaj ib tus nyob ze koj. Yog tias koj tsis muaj lub zej zog lab zoo li qhov no hauv koj thaj chaw, koj yuav tsum nqis peev qee yam khoom siv yooj yim rau cov txheej txheem no xws li:

1 ~ Tus lej digital rau ntsuas koj cov khoom xyaw kom raug

2 ~ Lub tshuab ua kom siab kom tsis muaj menyuam koj cov xov xwm nrog cua sov thiab ua pa

3 ~ Petri cov tais diav, tuaj yeem yuav online zoo nkauj pheej yig, iav rov siv tau lossis yas pov tseg yuav ua haujlwm tab sis rov siv tau yog ib txwm zoo dua:)

4 ~ Qee khob iav rau sib xyaw koj cov khoom xyaw hauv, ua kom tsis muaj menyuam, thiab khaws cia hauv.

Yog tias koj tsis xav nqis peev hauv cov cuab yeej ntawd koj tuaj yeem pom ua ntej nchuav malt extract agar daim hlau los yuav hauv cov vev xaib zoo li Amazon ~ nws tsuas yog yuav kim dua nyob rau lub sijhawm ntev yog tias koj npaj yuav ua txuas ntxiv ua haujlwm microbiological.

Muaj ntau ntau cov lus qhia zoo thiab youtube cov vis dis aus hais txog yuav ua li cas ua koj tus kheej cov khoom noj muaj txiaj ntsig zoo petri tais hauv tsev. Yog li ntau dua mus rau qhov nthuav dav ntawm qhov no, Kuv tsuas yog txuas rau ob peb - thiab muab daim ntawv qhia rau 2% Malt Extract Agar, uas yog cov khoom noj uas koj yuav siv.

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Malt Extract Agar Cov Ntaub Ntawv Cov Ntaub Ntawv

2% Malt Extract Agar (MEA) ~~ Daim ntawv qhia rau 1 Litre:

20g Malt Extract (ntau npias ua khoom, tuaj yeem yuav online)

15g Agar (tus sawv cev ua kom khov - qhov no yog siv los ua noj ntau zaus yog li nws tuaj yeem yuav online lossis ntawm qee lub khw muag khoom noj)

1 Litre H20 (Kuv ib txwm siv Cov Dej Rov Qab Osmosis uas tuaj yeem yuav tau ntawm cov khw muag khoom feem ntau hauv cov thoob nkas loos)

Daim ntawv qhia no tuaj yeem hloov kho tau yooj yim yog tias koj xav ua kom me dua. Kuv nquag ua tsuas yog 250 ml ntawm ib lub sijhawm piv txwv, yog li kuv tsuas yog siv 5 grams Malt Extract, 3.75 grams Agar, thiab 250 mL dej.

Kauj Ruam 3: Txhaum Koj Cov Petri Dish Nrog Fungi

Cov nceb uas koj pom tau hu ua "lub cev ua txiv" nws tsuas yog ib feem me me ntawm cov kab mob. Feem ntau ntawm nws lub cev tau muab zais rau hauv tsob ntoo (lossis hauv av), siv ntoo. Ib feem ntawm cov nceb no hu ua mycelium. Txhawm rau kev coj noj coj ua Schizophyllum Cov neeg dawb dawb, tsis muaj zog, rhizomatic, lub cev mycelial mus rau lub tais petri, koj yuav coj tus qauv ntawm cov qia, kom tsis muaj menyuam, thiab tso rau ntawm koj daim phiaj MEA. Mycelium yuav loj hlob los ntawm cov kab mob ua kom tsis muaj zog qia cov tub ntxhais coj mus kuaj thiab ua kom zoo rau lub tais zaub mov, zoo li lub cottony dawb.

Koj yuav tsum tau:

• Cov ntawv Malt Extract Agar (MEA) koj tau npaj hauv cov kauj ruam dhau los
• 70% Isopropyl cawv (rub cawv) thiab lub raj mis tsuag
• Ntawv phuam
• Scalpel, lossis tsuas yog rab chais hlau tsis huv - lub thawv txiav yuav ua haujlwm
• hlau tweezers
• hnab looj tes
• Nplaim taws - Kuv siv lub teeb alcohaul rau kom tsis muaj menyuam ntawm cov twj paj nruag, NonDisjunction muaj cov lus qhia zoo txog yuav ua li cas nyob ntawm no.
• Cov menyuam tsis muaj menyuam los txiav (Kuv tsuas yog siv cov zaub mov tsis huv rau qhov no)
• Parafilm, zaj duab xis paraffin siv los ntim cov tais diav petri - koj kuj tseem tuaj yeem siv daim npog ntsej muag yog tias koj tsis muaj qhov no

STERILE TECHNIQUE:

Qhov tseem ceeb koj xav kom nco ntsoov thaum sim siv cov txheej txheem kom tsis muaj menyuam yog cov kab mob thiab cov kab mob tsis tu ncua nyob hauv huab cua, yog li koj xav khaws koj cov tais diav petri kom ntau li ntau tau. Thaum koj nqa lub hau kom nchuav cov xov xwm, lossis ua kom tsis zoo, nws yog qhov zoo tshaj plaws los tuav lub hau tsuas yog saum lub tais, tso cai rau koj nkag mus rau sab hauv kab rov tav, tab sis khaws lub hau saum lub tais kom tiv thaiv kab mob los ntawm kev poob qis. yog ib pawg neeg loj heev ntawm tib neeg, kab mob, fungal, thiab lwm yam kab mob ~~ txawm hais tias koj tsis pom nws, koj nyob ib puag ncig los ntawm huab huab ntawm cov kab mob me me uas nthuav tawm ntawm koj lub cev. Ntau dua li tsuas yog zaum ntawm koj daim tawv nqaij, koj lub micro-biome nthuav tawm mus rau saum huab cua ~~ sooooooo, tsis txhob yaum koj txhais tes hla lub tais petri, lossis khoov dua lub tais qhib los saib hauv. Thiab hnav hnab looj tes yog tias koj muaj lawv;)

Teem Koj Qhov Chaw Ua Haujlwm SEMI-STERILE

Xaiv qhov chaw ua haujlwm tsis ntxeem tau ntawm cov khib nyiab thiab cov khoom pov tseg. Txau thaj tsam nrog koj li 70% Isopropyl Alcohol solution thiab so nws nrog daim ntawv so tes. Muab koj lub nplaim taws tso rau hauv nruab nrab ntawm koj thaj chaw ua haujlwm thiab ua kom pom kev. Koj yuav xav ua haujlwm ze rau nplaim taws ntau li ntau tau vim tias nws tsim qhov chaw me me kom tsis muaj menyuam nyob ib puag ncig nws. Muab koj daim phiaj MEA tso rau ib sab thiab koj cov qauv ua nceb mus rau qhov txiav tsis muaj menyuam ntawm lwm sab. Kuv ib txwm siv cov zaub mov tsis huv uas tsis muaj kab mob raws li kuv txiav tsis muaj menyuam nrog rau cov cuab yeej so. Ncuav ib co dej cawv Isopropyl rau hauv lub khob me me - koj yuav siv qhov no kom tsis muaj menyuam koj cov hniav thiab tus tweezers thaum ua haujlwm.

*¡~ INOCULATE ~!*

Ua ntej ua ib yam dab tsi, ua kom tsis huv koj cov hniav thiab tweezers los ntawm kev muab lawv tso rau hauv isopropyl alch thiab tom qab ntawd tuav lawv hla cov nplaim taws me ntsis. Tom qab ua kom tsis muaj menyuam, so lawv ntawm koj qhov tsis muaj menyuam yog tias koj tso lawv tseg. Siv tus tweezers, tuav cov nceb qia tseem thiab txiav ib feem ntawm nws. Nqa cov ntu no thiab txiav txhua sab kom koj xaus nrog lub voos xwmfab me me ntawm cov qia qia nrog txhua qhov sab nrauv tshem tawm. Qhov no pab tshem tawm txhua yam kab mob me me uas nyob ntawm cov nceb kom lawv tsis txhob kis kab mob rau koj. Tom qab ntawd dov lub hauv paus qia rau hauv isopropyl li ntawm 30 vib nas this, rub tawm, thiab cia kom qhuav hauv qhov chaw tsis huv ntawm cov nplaim taws (tsis txhob siv nplaim taws kom qhuav, qhov no yuav tua cov nceb). Nplaim taws kom tsis muaj menyuam rau koj tus tweezers dua thaum koj tos rau cov hauv paus qia kom qhuav. Tom qab ntawd yooj yim nqa lub hau ntawm ib qho ntawm koj daim ntawv MEA tsuas yog txaus kom xaub tus tweezers nkag los ntawm sab, thiab siv tus txhuam hniav kom tsis muaj menyuam tso rau hauv lub hauv paus ntawm lub phaj. Kaw lub phaj nrog daim kab xev, thiab dhau ob peb lub asthiv tom ntej, koj yuav tsum pom S. Commune mycelium colonizing phaj.

Kuv ib txwm ua ntau daim hlau thaum ua haujlwm nrog cov qauv tsiaj qus yog li yog tias kuv kis tau tus kab mob, kuv yuav muaj tsawg kawg yog ib kab lis kev cai huv.