Cov txheej txheem:
- Kauj Ruam 1: Kev Ua Yeeb Yaj Kiab
- Kauj Ruam 2: Lub Hom Phiaj
- Kauj Ruam 3: Sau Cov Khoom Siv no
- Kauj Ruam 4: Ua Ntawv Chromatography & Tsim Qauv
- Kauj Ruam 5: Sib sau Cov Khoom Siv Hluav Taws Xob
- Kauj Ruam 6: Sib Sau Cov Ntsuas
- Kauj Ruam 7: Program Qhov Ntsuas
- Kauj Ruam 8: Ntsuas Qhov Ntsuas
- Kauj Ruam 9: Kev txhim kho
- Kauj ruam 10: Cov neeg ua tim khawv
Video: Ntawv Chromatography/UV-Vis Kev sim nrog Arduino: 10 Kauj Ruam
2024 Tus sau: John Day | [email protected]. Kawg hloov kho: 2024-01-30 09:25
Qhov kev sim no siv Arduino microprocessor, nrog rau cov khoom siv hauv tsev, los ua ib qho kev sim ntawv chromatography thiab txheeb xyuas cov txiaj ntsig uas siv cov txheej txheem zoo ib yam li Ultraviolet-Visible (UV-Vis) spectroscopy. Qhov kev sim no yog xav kom rov ua dua ntau yam ntawm HPLC (Kev Ua Tau Zoo Ua Ntej Chromatography) ntsuas, xws li kev sib cais ntawm chromatographic thiab kev kuaj pom UV-Vis. Koj yuav kawm ntau yam txuj ci txuj ci nrog rau qhov kev sim no, ntxiv rau kawm txog Arduino microprocessor.
Kauj Ruam 1: Kev Ua Yeeb Yaj Kiab
Kauj Ruam 2: Lub Hom Phiaj
Lub hom phiaj ntawm qhov kev sim no yog rov ua qee cov haujlwm ntawm HPLC ntsuas. HPLC cais cov sib txuas los ntawm cov kua chromatography thiab siv UV-Vis ua lub ntsuas. Hauv qhov kev sim no, ob txoj haujlwm no yuav ua tiav cais. Daim ntawv chromatography yuav sawv cev rau cov kua chromatography hauv HPLC thiab yuav siv los cais cov khoom sib tov sib xyaw. Cov xim sib cais tom qab ntawd yuav raug siv los tsim cov qauv uas yuav raug tshuaj xyuas siv cov txheej txheem zoo ib yam li UV-Vis spectroscopy. Ib qho yooj yim dua ntawm qhov ntsuas UV-Vis yuav raug tsim, thiab qhov no yuav sawv cev rau lub ntsuas ntawm HPLC. Nrog rau qhov kev sim no, koj yuav kawm paub txog chromatography, UV-Vis spectroscopy, HPLC ntsuas lub zog, thiab Arduino Uno microprocessor.
Kauj Ruam 3: Sau Cov Khoom Siv no
Cov Khoom Siv Chromatography:
- Ntawv phuam (~ $ 1-2 toj ib yob)
- Cov tshuaj txhuam hniav (~ $ 3 toj ib lub thawv)
- Cov zaub mov xim (~ $ 4 toj ib lub thawv)
- Isopropyl (rub) cawv (~ $ 3 rau ib lub raj mis)
- Stapler
- Mem hluav
- Tus kav
- Khob
- Dej
- Txiab
- Yas qhwv
Cov khoom siv Arduino:
- Arduino Uno lossis microprocessor zoo sib xws (~ $ 15)
- Photoresistor
- Resistor (10K ohms) Cov
- Cov xov (txiv neej-txiv neej)
- Cov qhob cij (~ $ 5)
Cov Khoom Siv Ntsuas:
- Teeb nyem
- Qee hom iav ntshiab iav - iav koob txhaj tshuaj siv hauv qhov piv txwv no
- Styrofoam daim nrog qhov nyob hauv nruab nrab
- Daim ntawv tso quav tso quav
- Kab xev kab xev
Kauj Ruam 4: Ua Ntawv Chromatography & Tsim Qauv
Ntawv chromatography:
- Txiav ib puag ncig 4x6 nti duab plaub tawm ntawm daim phuam ntawv.
- Siv tus xaum thiab tus pas ntsuas, kos kab ncaj nraim mus rau qhov ntev dua ntawm daim phuam ntawv 1 nti ntawm hauv qab.
- Siv tus xaum, kos Xs raws kab no ntxhib 1/2 txog 3/4 ntiv tes sib nrug.
- Tsim cov khoom sib xyaw xim xim (xiav+daj, xiav+liab, liab+daj).
- Siv tus pas txhuam hniav, teev cov xim zaub mov sib xyaw thiab cov xim zaub mov ntshiab mus rau Xs kos. Txhua qhov xim lossis sib xyaw yuav tau sau rau ntawm nws tus kheej X. Cia kom qhuav.
- Yob daim ntawv so tes rau hauv lub tog raj kheej, nqa ob sab luv ua ke. Kaw lub tog raj kheej no ua ke, tawm qhov sib txawv me me ntawm ob sab ntawm daim ntawv phuam.
- Ntxiv kwv yees li 1/4 nti dej rau hauv lub khob uas yuav haum rau lub tog raj kheej uas koj tsim.
- Muab lub tog raj kheej tso rau hauv lub khob nrog lub dotted sab ze rau dej.
- Koj yuav pom cov dej tau nqus mus rau hauv daim phuam ntawv, thiab cov xim zaub mov yuav pib taug kev los ntawm daim phuam ntawv.
- Thaum cov kab dej ntawm daim phuam ntawv mus txog li 3/4 ntiv tes los ntawm sab saud, tshem daim ntawv so tes los ntawm lub khob. Tshem tawm cov ntaub ntawv thiab cia kom qhuav ncaj ntawm lwm daim phuam.
Tsim cov qauv:
- Thaum daim ntawv so tes qhuav, txiav tawm cov xim sib txawv los ntawm ob qho sib xyaw thiab cov zaub mov dawb huv.
- Ntxiv cov ntawv txiav tawm no rau isopropyl (rubbing) cawv.
- Npog qhov no nrog cov yas qhwv thiab tso kom ntub kom txog thaum feem ntau ntawm cov xim tau raug tshem tawm ntawm daim ntawv so tes.
- Cov no yuav yog cov piv txwv uas yuav tshuaj xyuas siv UV-Vis spectroscopy.
Kauj Ruam 5: Sib sau Cov Khoom Siv Hluav Taws Xob
Ua raws daim duab qhia chaw thiab daim duab teeb tsa lub rooj, xaim lub rooj mov ci rau Arduino.
Koj yuav siv cov hauv qab no ntawm Arduino:
- 5 V tso zis
- Hauv av
- A0 tso zis
Koj yuav siv cov hauv qab no:
- Txiv neej-txiv neej cov xov hlau
- 10 K ohm resistor
- Photoresistor
Kauj Ruam 6: Sib Sau Cov Ntsuas
-
Tsim tus qauv tuav
- Siv ib daim ntawm styrofoam nrog lub qhov nyob hauv nruab nrab loj txaus los tuav koj cov qauv.
- Poke qhov hla los ntawm ib leeg hauv ob sab ntawm styrofoam loj txaus los ua lub tsev photoresistor. Lwm qhov yuav yog lub teeb nkag.
- Teeb qhov no ntawm lub rooj tsavxwm nrog photoresistor hauv ib qho ntawm qhov.
-
Tsim lub raj los thaiv qhov pom kev ib puag ncig
- Siv daim ntawv tso quav tso quav thiab xev kab xev kaw qhov kawg.
- Qhov no yuav zaum hla tus tuav tus qauv thaum ntsuas ntsuas kom txo qhov tsis pom kev.
Kauj Ruam 7: Program Qhov Ntsuas
- Siv cov cai muab (UV_Vis_readings).
- Txheeb xyuas cov cai.
- Tshaj tawm cov cai rau Arduino.
- Txheeb xyuas tias lub luag haujlwm saib xyuas lub luag haujlwm tau ua haujlwm los ntawm kev pom yog tias muaj cov lej loj dua thaum lub photoresistor raug rau lub teeb thiab cov lej me dua thaum tus tiv thaiv nyob hauv qhov tsaus ntuj.
Kauj Ruam 8: Ntsuas Qhov Ntsuas
- Muab cov cawv isopropyl tso rau hauv lub raj iav lossis lub raj mis.
- Muab lub raj tso rau hauv tus qauv tuav, ua kom ntseeg tau tias nws ua kab nrog lub qhov hauv styrofoam.
- Tso lub teeb nyem nrog lub teeb nkag mus rau ib qho ntawm lub qhov.
- Muab daim ntawv tso quav tso rau saum kom thaiv qhov pom kev ntxiv.
- Qhib SerialMonitor thiab sau qhov ntsuas ib zaug ruaj khov.
- Tus nqi no yog kev sib kis, tab sis xav tau hloov dua siab tshiab.
- Muab tus nqi sib npaug los ntawm (5/1024) kom tau txais qhov sib kis tau tiag tiag (T).
- Ua cov lej hauv qab no kom tau txais kev nqus: nqus = log (1/T).
- Qhov no yog tus nqi ntawm qhov khoob.
- Rov ua cov kauj ruam 1-8 rau txhua tus qauv sib cais.
- Rho tawm qhov nqus ntawm qhov khoob los ntawm cov txiaj ntsig no los suav rau lub teeb tom qab.
- Sib piv qhov nqus dej - Koj puas pom muaj kev hloov pauv? Puas yog qhov khaus khaus ntau dua lossis qis dua hauv kev nqus?
Kauj Ruam 9: Kev txhim kho
Cov ntaub ntawv sib txawv:
- Cov lim dej kas fes yuav yog qhov hloov pauv tau zoo rau cov phuam ntawv.
- Lub teeb LED tuaj yeem ua haujlwm rau hauv txoj cai siv los ua lub teeb ci, tsis txhob siv lub teeb nyem.
- Cov raj ntsuas tuaj yeem siv qhov chaw ntawm lub khob iav.
Txhim kho kev sib cais:
Cov kuab tshuaj sib txawv tuaj yeem siv tau thaum ntawv chromatography los txhim kho kev sib cais ntawm cov xim zaub mov. Qhov no tuaj yeem ntsuas los ntawm kev pom cov kuab tshuaj dab tsi ua rau sib cais cov xim hauv cov xim sib xyaw zaub mov kom pom tseeb dua. Qhov sib piv sib txawv ntawm cov kuab tshuaj sib tov kuj tseem tuaj yeem sim
Ntau daim ntawv thov:
- Ib qho kev sim zoo sib xws tuaj yeem ua los ntawm kev cais cov xim los ntawm cov nroj tsuag.
- Lwm yam tshuaj muaj xim tuaj yeem kuaj, ib yam.
Kauj ruam 10: Cov neeg ua tim khawv
Kev tshoov siab rau txoj haujlwm no tau los ntawm cov hauv qab no:
www.purdue.edu/science/science-express/lab…
www.scientificamerican.com/article/chromat…
Kev tshoov siab rau teeb tsa pawg thawj coj saib thiab cov cai los ntawm:
www.instructables.com/id/How-to-use-a-phot…
create.arduino.cc/projecthub/Ayeon0122/rea…
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